Journal: BMC Microbiology
Article Title: Signatures in in vitro infection of NSC-34 mouse neurons and their cell nucleus with Rickettsia helvetica
doi: 10.1186/s12866-023-02859-0
Figure Lengend Snippet: Photomicrograph of R. helvetica -infected NSC-34 cells after 12 days of infection in nutrient-reduced D-medium. (A-C original magnification x 1000.) F-actin was stained with phalloidin ( A ), and presence of R. helvetica visualized by anti-rickettsial rabbit antiserum and FITC-labelled anti-rabbit immunoglobulin ( B ). In the merged image ( C ), the position of the green, fluorescent FITC-labeled bacteria can be seen next to small red stained actin nodes within the actin network and with close-up view (magnified by 120%) in image ( D ). Result is shown for one representative experiment, performed five times. Bars Fig. 2A-D denote 5 μm
Article Snippet: The same wash was repeated before incubation with phalloidin (1:40, Cat. no. A12380; Thermofisher Scientific,) for 10 min at room temperature.
Techniques: Infection, Staining, Labeling